1. Where should the alcohol treatment, chemical sterilization, and sterile-water rinsing of explants be performed?
2. A laboratory needs to prepare one litre of MS medium from stored nutrient stocks. Which procedure is appropriate?
3. Why are concentrated stock solutions used when preparing plant tissue culture media?
What is plant tissue culture?
The culture of plant seeds, organs, explants, tissues, cells, or protoplasts on nutrient media under sterile conditions.
What do in vitro culture and micropropagation have in common?
They involve growing plant material on artificial nutrient media under aseptic and controlled conditions.
What is plant tissue culture used for by commercial growers?
Asexual propagation to produce large quantities of clones of a particular plant.
Who is recognized as the father of plant tissue culture?
Gottlieb Haberlandt.
Which plant parts can be cultured using plant tissue culture?
Stems, leaves, roots, flowers, tissues, cells, and protoplasts.
Who proposed culturing isolated plant cells in vitro in 1902?
Haberlandt proposed culturing isolated plant cells in vitro in 1902.
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