Flashcard: Neural Circuit Imaging and Manipulation Techniques — 18 carte

Animal models — purpose?

Study neural circuits in vivo.

Transgenic animals — role?

Enable targeted genetic manipulation.

DNA recombinase — example?

Cre-lox system.

Viral vectors — advantage?

Targeted gene delivery in specific neurons.

Optogenetics — mechanism?

Control neurons with light-sensitive proteins.

Chemogenetics — method?

Use ligand-activated receptors to modulate activity.

Electrophysiology — technique?

Record neural activity via electrical signals.

Calcium during AP — influx?

Occurs during repolarization phase.

GECIs — purpose?

Fluorescently report calcium levels.

Single-photon microscopy — depth?

Limited to ~200 micrometers.

Two-photon microscopy — advantage?

Deeper imaging up to 1 mm.

Jablonski diagram — shows?

Excitation and emission processes.

Excitation wavelength — in two-photon?

Near-infrared, longer wavelengths.

Scattering — effect?

Limits resolution and depth in optical imaging.

Microendoscopes — use?

Access deep brain regions for imaging.

GRIN lenses — function?

Optical access to deep structures.

Calcium indicator variants — example?

GCaMP6s (slow), GCaMP6f (fast).

Optical imaging — limitation?

Trade-off between depth, resolution, and complexity.