Flashcards: Sterilization Techniques and Principles — 14 cards

Sterilization — definition?

Kills all microorganisms, including spores.

Sterilization methods — classification?

Physical and chemical methods.

Dry heat sterilization — method?

Uses hot air or flame to kill microbes.

Moist heat sterilization — method?

Uses steam under pressure, e.g., autoclaving.

Physical sterilization techniques — examples?

Heat, radiation, filtration.

Moist heat methods — example?

Autoclaving at 121°C.

Dry heat methods — example?

Hot air oven at 160–170°C.

Autoclaving — temperature?

121°C at 15 psi for 15 minutes.

Pasteurization — purpose?

Reduces pathogens in liquids.

Tyndallization — process?

Intermittent heating over 3 days.

Incineration — use?

Burning waste to ash.

Flaming — application?

Sterilize small heat-resistant instruments.

Biological indicators — example?

Bacillus atrophaeus spores for dry heat.

Sterilization vs disinfection?

Sterilization kills all, disinfection reduces microbes.