What is microscopy resolution?
The minimum distance at which two points remain distinguishable as separate.
What is the unaided human eye’s approximate resolving limit?
Approximately 100–200 µm.
What is the best resolution of an optical microscope?
About 200 nm (0.2 µm).
What is the first tissue-preparation step for bright-field microscopy?
Fixation.
How can fluorescence microscopy locate a target molecule?
A target-specific antibody binds it and is associated with a fluorochrome that emits visible light under ultraviolet excitation.
What prevents air molecules from colliding with electrons in an electron microscope?
An absolute vacuum.
Why can electron microscopy not image living cells?
The specimen must be dead in the vacuum column.
What does a transmission electron microscope (TEM) pass through a specimen to form an image?
Electrons pass through an ultrathin specimen.
How do heavy-metal salts create contrast in TEM images?
They accumulate in cellular material, making those regions dark while electron-transmitting regions appear light.
What colors do fluorescein and rhodamine emit, respectively?
Green and red.
Answer 11 questions on Cell Biology Techniques.
1. What does microscopy resolution describe?
2. A researcher observes a cell dividing and producing two new cells; which principle of cell theory does this illustrate?
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